dsred express Search Results


95
TaKaRa dsred express
Dsred Express, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
Addgene inc pmxs with dsred
(a) Cell proliferation. The number of SPRR1A-transduced PK-1 cells was counted every 3–4 days after transduction. (b) Cell proliferation assay. The number of viable cells was assessed at days 0, 1 and 3 by measuring cellular ATP levels. (c) Chemo-resistance to Gem. The cell viability in the presence of Gem was calculated as a percentage of the viability in its absence. (d) The mRNA expression of EMT markers was assessed by quantitative PCR. The expression was normalized to that of β-actin (ACTB). (e) Representative images of wound healing assay at 0, 12, 24, 36 and 48 h. (f) The effects of SPRR1A overexpression on the migration ability were determined by a wound-healing assay. The migration area (MA) in each group was calculated using the Image J software program, according to the following equation: MA = the area of the scratch at 0 h (A, A’)–the area of the scratch at 24 h (B, B’). The MA value of the <t>pMXs-</t> <t>DsRed</t> population was used as a reference. The following equation determined the relative cell migration ability: Relative cell migration ability = MA (pMXs- SPRR1A ) / MA (pMXs- DsRed ). N.D., not detected; n.s., not significant.
Pmxs With Dsred, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Becton Dickinson rnai-ready psiren-retroq-dsred-express retrovirus vector
(a) Cell proliferation. The number of SPRR1A-transduced PK-1 cells was counted every 3–4 days after transduction. (b) Cell proliferation assay. The number of viable cells was assessed at days 0, 1 and 3 by measuring cellular ATP levels. (c) Chemo-resistance to Gem. The cell viability in the presence of Gem was calculated as a percentage of the viability in its absence. (d) The mRNA expression of EMT markers was assessed by quantitative PCR. The expression was normalized to that of β-actin (ACTB). (e) Representative images of wound healing assay at 0, 12, 24, 36 and 48 h. (f) The effects of SPRR1A overexpression on the migration ability were determined by a wound-healing assay. The migration area (MA) in each group was calculated using the Image J software program, according to the following equation: MA = the area of the scratch at 0 h (A, A’)–the area of the scratch at 24 h (B, B’). The MA value of the <t>pMXs-</t> <t>DsRed</t> population was used as a reference. The following equation determined the relative cell migration ability: Relative cell migration ability = MA (pMXs- SPRR1A ) / MA (pMXs- DsRed ). N.D., not detected; n.s., not significant.
Rnai Ready Psiren Retroq Dsred Express Retrovirus Vector, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Institut Curie dsred-isce1 expression plasmid
(a) Cell proliferation. The number of SPRR1A-transduced PK-1 cells was counted every 3–4 days after transduction. (b) Cell proliferation assay. The number of viable cells was assessed at days 0, 1 and 3 by measuring cellular ATP levels. (c) Chemo-resistance to Gem. The cell viability in the presence of Gem was calculated as a percentage of the viability in its absence. (d) The mRNA expression of EMT markers was assessed by quantitative PCR. The expression was normalized to that of β-actin (ACTB). (e) Representative images of wound healing assay at 0, 12, 24, 36 and 48 h. (f) The effects of SPRR1A overexpression on the migration ability were determined by a wound-healing assay. The migration area (MA) in each group was calculated using the Image J software program, according to the following equation: MA = the area of the scratch at 0 h (A, A’)–the area of the scratch at 24 h (B, B’). The MA value of the <t>pMXs-</t> <t>DsRed</t> population was used as a reference. The following equation determined the relative cell migration ability: Relative cell migration ability = MA (pMXs- SPRR1A ) / MA (pMXs- DsRed ). N.D., not detected; n.s., not significant.
Dsred Isce1 Expression Plasmid, supplied by Institut Curie, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Becton Dickinson rnai-ready psiren-dnr-dsred-express vector
(a) Cell proliferation. The number of SPRR1A-transduced PK-1 cells was counted every 3–4 days after transduction. (b) Cell proliferation assay. The number of viable cells was assessed at days 0, 1 and 3 by measuring cellular ATP levels. (c) Chemo-resistance to Gem. The cell viability in the presence of Gem was calculated as a percentage of the viability in its absence. (d) The mRNA expression of EMT markers was assessed by quantitative PCR. The expression was normalized to that of β-actin (ACTB). (e) Representative images of wound healing assay at 0, 12, 24, 36 and 48 h. (f) The effects of SPRR1A overexpression on the migration ability were determined by a wound-healing assay. The migration area (MA) in each group was calculated using the Image J software program, according to the following equation: MA = the area of the scratch at 0 h (A, A’)–the area of the scratch at 24 h (B, B’). The MA value of the <t>pMXs-</t> <t>DsRed</t> population was used as a reference. The following equation determined the relative cell migration ability: Relative cell migration ability = MA (pMXs- SPRR1A ) / MA (pMXs- DsRed ). N.D., not detected; n.s., not significant.
Rnai Ready Psiren Dnr Dsred Express Vector, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Becton Dickinson psiren-dnr-dsred-express
(a) Cell proliferation. The number of SPRR1A-transduced PK-1 cells was counted every 3–4 days after transduction. (b) Cell proliferation assay. The number of viable cells was assessed at days 0, 1 and 3 by measuring cellular ATP levels. (c) Chemo-resistance to Gem. The cell viability in the presence of Gem was calculated as a percentage of the viability in its absence. (d) The mRNA expression of EMT markers was assessed by quantitative PCR. The expression was normalized to that of β-actin (ACTB). (e) Representative images of wound healing assay at 0, 12, 24, 36 and 48 h. (f) The effects of SPRR1A overexpression on the migration ability were determined by a wound-healing assay. The migration area (MA) in each group was calculated using the Image J software program, according to the following equation: MA = the area of the scratch at 0 h (A, A’)–the area of the scratch at 24 h (B, B’). The MA value of the <t>pMXs-</t> <t>DsRed</t> population was used as a reference. The following equation determined the relative cell migration ability: Relative cell migration ability = MA (pMXs- SPRR1A ) / MA (pMXs- DsRed ). N.D., not detected; n.s., not significant.
Psiren Dnr Dsred Express, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
rocky mountain labs dsred-expressing brucella abortus strain 2038
(a) Cell proliferation. The number of SPRR1A-transduced PK-1 cells was counted every 3–4 days after transduction. (b) Cell proliferation assay. The number of viable cells was assessed at days 0, 1 and 3 by measuring cellular ATP levels. (c) Chemo-resistance to Gem. The cell viability in the presence of Gem was calculated as a percentage of the viability in its absence. (d) The mRNA expression of EMT markers was assessed by quantitative PCR. The expression was normalized to that of β-actin (ACTB). (e) Representative images of wound healing assay at 0, 12, 24, 36 and 48 h. (f) The effects of SPRR1A overexpression on the migration ability were determined by a wound-healing assay. The migration area (MA) in each group was calculated using the Image J software program, according to the following equation: MA = the area of the scratch at 0 h (A, A’)–the area of the scratch at 24 h (B, B’). The MA value of the <t>pMXs-</t> <t>DsRed</t> population was used as a reference. The following equation determined the relative cell migration ability: Relative cell migration ability = MA (pMXs- SPRR1A ) / MA (pMXs- DsRed ). N.D., not detected; n.s., not significant.
Dsred Expressing Brucella Abortus Strain 2038, supplied by rocky mountain labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Jackson Laboratory pc3-dsred-express cells
(a) Cell proliferation. The number of SPRR1A-transduced PK-1 cells was counted every 3–4 days after transduction. (b) Cell proliferation assay. The number of viable cells was assessed at days 0, 1 and 3 by measuring cellular ATP levels. (c) Chemo-resistance to Gem. The cell viability in the presence of Gem was calculated as a percentage of the viability in its absence. (d) The mRNA expression of EMT markers was assessed by quantitative PCR. The expression was normalized to that of β-actin (ACTB). (e) Representative images of wound healing assay at 0, 12, 24, 36 and 48 h. (f) The effects of SPRR1A overexpression on the migration ability were determined by a wound-healing assay. The migration area (MA) in each group was calculated using the Image J software program, according to the following equation: MA = the area of the scratch at 0 h (A, A’)–the area of the scratch at 24 h (B, B’). The MA value of the <t>pMXs-</t> <t>DsRed</t> population was used as a reference. The following equation determined the relative cell migration ability: Relative cell migration ability = MA (pMXs- SPRR1A ) / MA (pMXs- DsRed ). N.D., not detected; n.s., not significant.
Pc3 Dsred Express Cells, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Rainbow Transgenic Flies eyeless promoter controlling the expression of dsred-express
(a) Cell proliferation. The number of SPRR1A-transduced PK-1 cells was counted every 3–4 days after transduction. (b) Cell proliferation assay. The number of viable cells was assessed at days 0, 1 and 3 by measuring cellular ATP levels. (c) Chemo-resistance to Gem. The cell viability in the presence of Gem was calculated as a percentage of the viability in its absence. (d) The mRNA expression of EMT markers was assessed by quantitative PCR. The expression was normalized to that of β-actin (ACTB). (e) Representative images of wound healing assay at 0, 12, 24, 36 and 48 h. (f) The effects of SPRR1A overexpression on the migration ability were determined by a wound-healing assay. The migration area (MA) in each group was calculated using the Image J software program, according to the following equation: MA = the area of the scratch at 0 h (A, A’)–the area of the scratch at 24 h (B, B’). The MA value of the <t>pMXs-</t> <t>DsRed</t> population was used as a reference. The following equation determined the relative cell migration ability: Relative cell migration ability = MA (pMXs- SPRR1A ) / MA (pMXs- DsRed ). N.D., not detected; n.s., not significant.
Eyeless Promoter Controlling The Expression Of Dsred Express, supplied by Rainbow Transgenic Flies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Lonza dcx-dsred-express
(a) Cell proliferation. The number of SPRR1A-transduced PK-1 cells was counted every 3–4 days after transduction. (b) Cell proliferation assay. The number of viable cells was assessed at days 0, 1 and 3 by measuring cellular ATP levels. (c) Chemo-resistance to Gem. The cell viability in the presence of Gem was calculated as a percentage of the viability in its absence. (d) The mRNA expression of EMT markers was assessed by quantitative PCR. The expression was normalized to that of β-actin (ACTB). (e) Representative images of wound healing assay at 0, 12, 24, 36 and 48 h. (f) The effects of SPRR1A overexpression on the migration ability were determined by a wound-healing assay. The migration area (MA) in each group was calculated using the Image J software program, according to the following equation: MA = the area of the scratch at 0 h (A, A’)–the area of the scratch at 24 h (B, B’). The MA value of the <t>pMXs-</t> <t>DsRed</t> population was used as a reference. The following equation determined the relative cell migration ability: Relative cell migration ability = MA (pMXs- SPRR1A ) / MA (pMXs- DsRed ). N.D., not detected; n.s., not significant.
Dcx Dsred Express, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Qiagen expression plasmids encoding for crip1a-gfp and mglu8ar-dsred
(a) Cell proliferation. The number of SPRR1A-transduced PK-1 cells was counted every 3–4 days after transduction. (b) Cell proliferation assay. The number of viable cells was assessed at days 0, 1 and 3 by measuring cellular ATP levels. (c) Chemo-resistance to Gem. The cell viability in the presence of Gem was calculated as a percentage of the viability in its absence. (d) The mRNA expression of EMT markers was assessed by quantitative PCR. The expression was normalized to that of β-actin (ACTB). (e) Representative images of wound healing assay at 0, 12, 24, 36 and 48 h. (f) The effects of SPRR1A overexpression on the migration ability were determined by a wound-healing assay. The migration area (MA) in each group was calculated using the Image J software program, according to the following equation: MA = the area of the scratch at 0 h (A, A’)–the area of the scratch at 24 h (B, B’). The MA value of the <t>pMXs-</t> <t>DsRed</t> population was used as a reference. The following equation determined the relative cell migration ability: Relative cell migration ability = MA (pMXs- SPRR1A ) / MA (pMXs- DsRed ). N.D., not detected; n.s., not significant.
Expression Plasmids Encoding For Crip1a Gfp And Mglu8ar Dsred, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Jackson Laboratory transgenic mice expressing gfp or dsred under the control of the β-actin promoter
(a) Cell proliferation. The number of SPRR1A-transduced PK-1 cells was counted every 3–4 days after transduction. (b) Cell proliferation assay. The number of viable cells was assessed at days 0, 1 and 3 by measuring cellular ATP levels. (c) Chemo-resistance to Gem. The cell viability in the presence of Gem was calculated as a percentage of the viability in its absence. (d) The mRNA expression of EMT markers was assessed by quantitative PCR. The expression was normalized to that of β-actin (ACTB). (e) Representative images of wound healing assay at 0, 12, 24, 36 and 48 h. (f) The effects of SPRR1A overexpression on the migration ability were determined by a wound-healing assay. The migration area (MA) in each group was calculated using the Image J software program, according to the following equation: MA = the area of the scratch at 0 h (A, A’)–the area of the scratch at 24 h (B, B’). The MA value of the <t>pMXs-</t> <t>DsRed</t> population was used as a reference. The following equation determined the relative cell migration ability: Relative cell migration ability = MA (pMXs- SPRR1A ) / MA (pMXs- DsRed ). N.D., not detected; n.s., not significant.
Transgenic Mice Expressing Gfp Or Dsred Under The Control Of The β Actin Promoter, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(a) Cell proliferation. The number of SPRR1A-transduced PK-1 cells was counted every 3–4 days after transduction. (b) Cell proliferation assay. The number of viable cells was assessed at days 0, 1 and 3 by measuring cellular ATP levels. (c) Chemo-resistance to Gem. The cell viability in the presence of Gem was calculated as a percentage of the viability in its absence. (d) The mRNA expression of EMT markers was assessed by quantitative PCR. The expression was normalized to that of β-actin (ACTB). (e) Representative images of wound healing assay at 0, 12, 24, 36 and 48 h. (f) The effects of SPRR1A overexpression on the migration ability were determined by a wound-healing assay. The migration area (MA) in each group was calculated using the Image J software program, according to the following equation: MA = the area of the scratch at 0 h (A, A’)–the area of the scratch at 24 h (B, B’). The MA value of the pMXs- DsRed population was used as a reference. The following equation determined the relative cell migration ability: Relative cell migration ability = MA (pMXs- SPRR1A ) / MA (pMXs- DsRed ). N.D., not detected; n.s., not significant.

Journal: PLoS ONE

Article Title: Increased expression of SPRR1A is associated with a poor prognosis in pancreatic ductal adenocarcinoma

doi: 10.1371/journal.pone.0266620

Figure Lengend Snippet: (a) Cell proliferation. The number of SPRR1A-transduced PK-1 cells was counted every 3–4 days after transduction. (b) Cell proliferation assay. The number of viable cells was assessed at days 0, 1 and 3 by measuring cellular ATP levels. (c) Chemo-resistance to Gem. The cell viability in the presence of Gem was calculated as a percentage of the viability in its absence. (d) The mRNA expression of EMT markers was assessed by quantitative PCR. The expression was normalized to that of β-actin (ACTB). (e) Representative images of wound healing assay at 0, 12, 24, 36 and 48 h. (f) The effects of SPRR1A overexpression on the migration ability were determined by a wound-healing assay. The migration area (MA) in each group was calculated using the Image J software program, according to the following equation: MA = the area of the scratch at 0 h (A, A’)–the area of the scratch at 24 h (B, B’). The MA value of the pMXs- DsRed population was used as a reference. The following equation determined the relative cell migration ability: Relative cell migration ability = MA (pMXs- SPRR1A ) / MA (pMXs- DsRed ). N.D., not detected; n.s., not significant.

Article Snippet: Concomitantly, pMXs with DsRed (pMXs- DsRed ) (Addgene, Watertown, MA, USA; catalog number: 22724) was used as a control vector.

Techniques: Transduction, Proliferation Assay, Expressing, Real-time Polymerase Chain Reaction, Wound Healing Assay, Over Expression, Migration, Software